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KMID : 0379120030310020103
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Korean Journal of Mycology
2003 Volume.31 No. 2 p.103 ~ p.113
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Morphological and Molecular Characterization of Alternaria Isolates from Solanaceous Crops
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You Seoung-Hun
Park Myoung-Soo
Jo Hae-Sun
Kim Byoung-Ryun
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Abstract
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Twenty five isolates of Alternaria were obtained from various solanaceous crops in Korea. For all isolates, morphological characteristics of the conidia were determined and compared with those of representative isolates of A. solani and A. tomatophila. A selection of the isolates and the representative Alternaria isolates were evaluated for Pathogenicity to potato, tomato, egg plant and red pepper. Molecular characteristics of 17 isolates of Alternaria inculding the representative isolates were determined using sequence analysis of IRS rDNA and histone H3 gene, and URP-PCR analysis. Based on morphological characteristics, the isolates from the solanaceous crops were grouped as identical or very similar to either A. tomatophila (ATO), A. solani (ASO), and unidentified Alternaria sp. (ASP). Isolates of ASO were moderately pathogenic to all the solanaceous crops tested, but ATO isolates were highly pathogenic to tomato and the ASP isolate was pathogenic only to potato. Among the molecular markers used in this study, the URP-PCR analysis was found to be appropriate for taxonomic resolution of these species. Based on the conidial morphology, pathogenicity test and molecular characteristics, A. tomatophila (early blight of tomato) could be distinguished from A. solani (early blight of potato), and the Alternaria sp. (ASP) from potato, which was closely related to ASO in conidial morphology, was considered as a new species.
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KEYWORD
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Alternaria solani, A. tomatophila, Alternaria sp., Histone H3 gene, ITS, Morphology, Pathogenicity, Solanaceous crops, URP-PCR
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